FK1012

FK1012
Names
IUPAC name
(1R,12S,13R,14S,18E,21S,23S,24R,25R,27R)-1,14-Dihydroxy-17-{(2E)-4-[(12S,13R,14S,17R,18E,21S,23S,24R,25R,27R)-14-hydroxy-12-{(1E)-1-[(1R,3R,4R)-4-hydroxy-3-methoxycyclohexyl]-1-propen-2-yl}-23,25-dimethoxy-13,19,21,27-tetramethyl-2,3,10,16-tetraoxo-11,28-dioxa-4-azatricyclo[22.3.1.04,9]octacos-18-en-17-yl]-2-buten-1-yl}-12-{(1E)-1-[(1R,3R,4R)-4-hydroxy-3-methoxycyclohexyl]-1-propen-2-yl}-23,25-dimethoxy-13,19,21,27-tetramethyl-11,28-dioxa-4-azatricyclo[22.3.1.04,9]octacos-18-ene-2,3,10,16-tetrone
Identifiers
3D model (JSmol)
ChEMBL
ChemSpider
  • InChI=1S/C86H134N2O23/c1-47-33-49(3)37-71(104-13)79-73(106-15)41-53(7)78(108-79)75(95)82(97)87-31-21-19-25-61(87)84(99)109-76(51(5)39-57-27-29-63(89)69(43-57)102-11)55(9)65(91)45-67(93)59(35-47)23-17-18-24-60-36-48(2)34-50(4)38-72(105-14)80-74(107-16)42-54(8)86(101,111-80)81(96)83(98)88-32-22-20-26-62(88)85(100)110-77(56(10)66(92)46-68(60)94)52(6)40-58-28-30-64(90)70(44-58)103-12/h17-18,35-36,39-40,49-50,53-66,69-74,76-80,89-92,101H,19-34,37-38,41-46H2,1-16H3/b18-17+,47-35+,48-36+,51-39+,52-40+/t49-,50-,53+,54+,55+,56+,57-,58-,59+,60?,61?,62?,63+,64+,65-,66-,69+,70+,71-,72-,73+,74+,76+,77+,78?,79+,80+,86+/m0/s1
    Key: IOISFHIZCFPBTB-HSZXOJBTSA-N
  • C[C@@H]1C[C@@H]([C@@H]2[C@@H](C[C@H](C(O2)C(=O)C(=O)N3CCCCC3C(=O)O[C@@H]([C@@H]([C@H](CC(=O)[C@@H](/C=C(/C1)\C)C/C=C/CC4/C=C(/C[C@@H](C[C@@H]([C@@H]5[C@@H](C[C@H]([C@@](O5)(C(=O)C(=O)N6CCCCC6C(=O)O[C@@H]([C@@H]([C@H](CC4=O)O)C)/C(=C/[C@@H]7CC[C@H]([C@@H](C7)OC)O)/C)O)C)OC)OC)C)\C)O)C)/C(=C/[C@@H]8CC[C@H]([C@@H](C8)OC)O)/C)C)OC)OC
Properties
C86H134N2O23
Molar mass 1564.009 g·mol−1
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).

FK1012 is a dimer consisting of two molecules of tacrolimus (FK506) linked via their vinyl groups.[1] It is used as a research tool in chemically induced dimerization applications. FK1012 is a chemical inducer of dimerization (CID) which makes the protein capable of dimerization or oligomerization of fusion proteins containing one or more FKBP12 domains.[2] It is used in pharmacology to act as a mediator in the formation of FK506 dimer.[3] FK506 binding proteins (FKBPs) do not normally form dimers but can be caused to dimerize in the presence of FK1012. Genetically engineered proteins based on FKBPs can be used to manipulate protein localization, signaling pathways and protein activation.[4]

References

  1. ^ Steven T. Diver; Stuart L. Schreiber (1997). "Single-step syntheses of cell permeable protein dimerizers that activate signal transduction and gene expression". J. Am. Chem. Soc. 119 (22): 5106–5109. Bibcode:1997JAChS.119.5106D. doi:10.1021/ja963891c.
  2. ^ Keenan, Terence; Yaeger, David R.; Courage, Nancy L.; Rollins, Carl T.; Pavone, Mary Ellen; Rivera, Victor M.; Yang, Wu; Guo, Tao; Amara, Jane F.; Clackson, Tim; Gilman, Michael; Holt, Dennis A. (1998-08-01). "Synthesis and activity of bivalent FKBP12 ligands for the regulated dimerization of proteins". Bioorganic & Medicinal Chemistry. 6 (8): 1309–1335. doi:10.1016/S0968-0896(98)00125-4. ISSN 0968-0896. PMID 9784872.
  3. ^ Blau, C. Anthony; Peterson, Kenneth R.; Drachman, Jonathan G.; Spencer, David M. (1997). "A Proliferation Switch for Genetically Modified Cells". Proceedings of the National Academy of Sciences of the United States of America. 94 (7): 3076–3081. Bibcode:1997PNAS...94.3076B. doi:10.1073/pnas.94.7.3076. ISSN 0027-8424. JSTOR 41778. PMC 20324. PMID 9096348.
  4. ^ Fegan, A; White, B; Carlson, JC; Wagner, CR (Jun 9, 2010). "Chemically controlled protein assembly: techniques and applications". Chemical Reviews. 110 (6): 3315–36. doi:10.1021/cr8002888. PMID 20353181.

Further reading

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